Figure 3
Expression of type 1 and type 2 tumour necrosis factor receptor (TNFR1/2) and macrophage colony-stimulating factor receptor (M-CSFR) on monocytes of patients with rheumatoid arthritis (RA) and healthy controls (HCs). Peripheral blood mononuclear cells were stained with immunoglobulin (Ig) G2a anti-TNFR1 antibody, rabbit IgG anti-M-CSFR polyclonal antibody, or isotype-matched control antibody, followed by fluorescein isothiocyanate (FITC)-conjugated anti-mouse IgG2a antibody or phycoerythrin-conjugated anti-rabbit IgG antibody, and with FITC-conjugated anti-TNFR2 antibody. TNFR1, TNFR2, and M-CSFR expression on monocytes from patients with RA and HCs was analysed by flow cytometric analysis. The intensity of cytokine receptor was expressed by the ratio of the mean fluorescence intensity (MFI) of staining with anti-cytokine receptor antibody to the MFI of control antibody. Values are the mean ± standard error of the mean. n, number of samples tested.