Figure 4
Induction of mRNA expression of type 1 and type 2 tumour necrosis factor receptor (TNFR1/2) in monocytes by interleukin-10 (IL-10). Adhered monocytes from patients with rheumatoid arthritis (RA) and healthy controls (HCs) (5 × 105 cells in culture medium with 10% fetal calf serum) were incubated for 12 hours with or without 25 ng/ml IL-10. Total cellular RNA was extracted from these monocytes, and mRNA expression was analysed by real-time polymerase chain reaction analysis as described in Materials and methods. Levels of TNFR1 and TNFR2 mRNAs were normalised relative to β-actin expression, and the ratio of the levels of monocytes incubated with IL-10 to the levels without IL-10 was determined. Values are the mean ± standard error of the mean. n, number of samples tested.