Figure 5
Cytokine receptor induction on monocytes stimulated with interleukin-10 (IL-10) and macrophage colony-stimulating factor (M-CSF). (a) Induction of monocyte expression of M-CSF receptor (M-CSFR) by IL-10 and/or M-CSF. Purified normal monocytes from different individuals (5 × 105 cells in culture medium with 10% fetal calf serum) were incubated for 3 days with or without 50 ng/ml M-CSF, 25 ng/ml IL-10, or IL-10 plus M-CSF. M-CSFR expression was analysed by flow cytometric analysis, and the cytokine-mediated M-CSFR induction was expressed by the ratio of the mean fluorescence intensity (MFI) of M-CSFR expression with cytokines to the MFI without cytokines. Values are the mean ± standard error of the mean. (b) Representative histographic patterns of type 1 and type 2 tumour necrosis factor receptor (TNFR1/2) expression on monocytes incubated with or without IL-10 plus M-CSF. TNFR1 and TNFR2 expression was analysed by flow cytometric analysis. (c) Induction of monocyte expression of TNFR1 and TNFR2 by IL-10 and/or M-CSF. The cytokine-mediated TNFR1/2 induction was expressed by the ratio of the mean fluorescence intensity (MFI) of TNFR1/2 expression with cytokines to the MFI without cytokines. n, number of samples tested.