Figure 7
Macrophage migration inhibitory factor (MIF) activates protein kinase C (PKC)δ. Rheumatoid arthritis (RA) synovial fibroblasts were stimulated with MIF (25 nM) for 1 minutes, 5 minutes, 15 minutes, 30 minutes, and 45 minutes. Western blots were performed with phospho-specific antibodies against different isoforms of PKC. *P-PKC (pan) (a), *p-PKCαI βII (b) and *p-PKCε (c) were not upregulated by MIF. On the other hand, MIF induced the phosphorylation of PKCδ at Thr505 (e), but not on Tyr311 (d). NS, non-stimulated; p-PKC, phosphor-protein kinase C; rhMIF, recombinant human macrophage migration inhibitory factor.