Figure 7
From: Mice expressing HLA-DQ6α8β transgenes develop polychondritis spontaneously
Matrilin-1-specific IgG present during spontaneous polychondritis in DQ6α8β transgenic mice. Representative sera from middle-aged mice displaying spontaneous polychondritis, from young naïve mice, from young mice with type II collagen (CII)-induced polychondritis or from middle-aged mice not displaying clinical disease were separated by SDS-PAGE electrophoresis and were blotted for the presence of matrilin-1-specific or CII-specific IgG. Although minor levels of matrilin-1-specific IgG were detectable in each sera that increased with age, it was highly elevated in each spontaneous polychondritis mouse examined. CII-specific IgG was only detected in the CII-immunized mouse, as also observed by ELISA.