Table 1 Primer sequences used to detect mRNA in mouse costal cartilage explants
Genes | Temperature (°C) | Genbank ID | Forward primer | Reverse primer | Amplicon length (bp) |
|---|---|---|---|---|---|
HPRT | 58 | NM_013556 | 5'-gctggtgaaaaggacctct-3' | 5'-cacaggactagaacacctgc-3' | 249 |
COX-1 | 58 | BC005573 | 5'-ctttgcacaacacttcacccacc-3' | 5'-agcaacccaaacacctcctgg-3' | 285 |
COX-2 | 58 | NM_011198 | 5'-gcattctttgcccagcactt-3' | 5'-agaccaggcaccgaccaaaga-3' | 299 |
COX-3 | 58 | AY547265 | 5'-tgaacgctaggctcaactctc-3' | 5'-ggttctggcacggatagtaac-3' | 349 |
cPGES | 58 | AY281130 | 5'-agtcatggcctaggttaac-3' | 5'-tgtgaatcatcatctgctcc-3' | 196 |
mPGES-1 | 58 | NM_022415 | 5'-ctgctggtcatcaagatgtacg-3' | 5'-cccaggtaggccacgtgtgt-3' | 294 |
mPGES-2 | 58 | BC004846 | 5'-aagacatgtcccttctgc-3' | 5'-ccaagatgggcactttcc-3' | 133 |
15-PGDH | 58 | NM_008278 | 5'-gccaaggtagcattggtggat-3' | 5'-cttccgaaatggtctacaact-3' | 164 |