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Figure 1 | Arthritis Research & Therapy

Figure 1

From: Characterisation of the immune response to type I collagen in scleroderma

Figure 1

T-Cell Proliferation to C1. (a) Peripheral blood mononuclear cell proliferation monitored using carboxy-fluorescein diacetate, succinimidyl ester (CFSE) labeling. Representative dot plot of CFSE/CD4 double-labeling demonstrates increased T-cell proliferation (CFSElow) in response to (in vitro culture with) type I collagen (CI) or β1,2 chain. (b) CI-reactive lymphocytes express an activated, memory phenotype. Representative histogram demonstrates that the majority of CFSElow (that is, antigen-specific proliferating T cells) express CD45RO and CD25 (right panels) compared with the CFSEhigh (non-responsive to antigen) T cells (left panels). Grey shaded areas represent staining with the appropriate isotype control. PBS, phosphate-buffered saline.

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