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Figure 3 | Arthritis Research & Therapy

Figure 3

From: Metalloproteinase and inhibitor expression profiling of resorbing cartilage reveals pro-collagenase activation as a critical step for collagenolysis

Figure 3

Profiling gelatinase gene expression and gelatinolytic activity in resorbing cartilage. Bovine nasal cartilage chips were cultured in medium ± interleukin-1 (IL-1) + oncostatin M (OSM) for 14 days exactly as described in the legend to Figure 1. RNA was extracted from cartilage, and matrix metalloproteinase (MMP)-2 and -9 gene expression determined by real-time polymerase chain reaction (n = 3) as described in Materials and methods. The data are presented relative to 18S. As a measure of gelatinase activity, the culture media were analysed by gelatin zymography. Values are the mean ± standard error of the mean. = control; ▲ = IL-1+OSM.

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