Profiling gelatinase gene expression and gelatinolytic activity in resorbing cartilage. Bovine nasal cartilage chips were cultured in medium ± interleukin-1 (IL-1) + oncostatin M (OSM) for 14 days exactly as described in the legend to Figure 1. RNA was extracted from cartilage, and matrix metalloproteinase (MMP)-2 and -9 gene expression determined by real-time polymerase chain reaction (n = 3) as described in Materials and methods. The data are presented relative to 18S. As a measure of gelatinase activity, the culture media were analysed by gelatin zymography. Values are the mean ± standard error of the mean. ◇ = control; ▲ = IL-1+OSM.