Table 1 Bovine metalloproteinase and inhibitor primers for real-time polymerase chain reaction
Gene | Sequence (5'-3') | Length (bp) |
|---|---|---|
MMP-1 | GATGCCGCTGTTTCTGAGGA | 372 |
| Â | GACTGAGCGACTAACACGACACAT | Â |
MMP-2 | TCTGCCCCCATGAAGCCCTGTT | 347 |
| Â | GCCCCACTTGCGGTCATCATCGTA | Â |
MMP-3 | TTAGAGAACATGGGGACTTTTTG | 360 |
| Â | CGGGTTCGGGAGGCACAG | Â |
MMP-8 | ATGCTGCTTATGAGGATTTTGACA | 101 |
| Â | GCCTGGGGTAACCTTGCTGAGTA | Â |
MMP-9 | CGCCACCACCGCCAACTACG | 350 |
| Â | GGGGGTGCTCCTCTGTGAATCTGT | Â |
MMP-12 | TGTGACCCCAATATGAGTTTT | 155 |
| Â | TTGAATGTAAGACGGTAGGTTT | Â |
MMP-13 | CCCTCTGGTCTGTTGGCTCAC | 304 |
| Â | CTGGCGTTTTGGGATGTTTAGA | Â |
MMP-14 | AGGCCGACATCATGATCTTCTTTG | 375 |
| Â | CTGGGTTGAGGGGGCATCTTAGTG | Â |
MMP-16 | ACCCCAGGATGTCAGTGC | 287 |
| Â | AATAGCTTTACGGGTTTCAGG | Â |
TIMP-1 | TGGGCACCTGCACATCACC | 277 |
| Â | CATCTGGGCCCGCAAGGACTG | Â |
TIMP-2 | ATAGTGATCAGGGCCAAAGCAGTC | 277 |
| Â | TGTCCCAGGGCACGATGAAGTC | Â |
TIMP-3 | GATGTACCGAGGATTCACCAAGAT | 356 |
| Â | GCCGGATGCAAGCGTAGT | Â |
TIMP-4 | ATATTTATACGCCTTTTGATTCCT | 297 |
| Â | GGTACCCGTAGAGCTTCCGTTCC | Â |
α2M | GCCCGCTTTGCCCCTAACA | 359 |
| Â | TCGTCCACCCCACCCTTGATG | Â |
RECK | GTAATTGCCAAAAAGTGAAA | 352 |
| Â | TAGGTGCATATAAACAAGAAGTA | Â |
ADAMTS-1 | GCTGCCCTCACACTGCGGAAC | 264 |
| Â | CATCATGGGGCATGTTAAACAC | Â |
ADAMTS-4 | GCGCCCGCTTCATCACTG | 101 |
| Â | TTGCCGGGGAAGGTCACG | Â |
ADAMTS-5 | AAGCTGCCGGCCGTGGAAGGAA | 196 |
| Â | TGGGTTATTGCAGTGGCGGTAGG | Â |
ADAMTS-8 | AGATCTTTGGGCTGGGCTTCC | 116 |
| Â | GGCTGGCATTCCTCGTGTGG | Â |
ADAMTS-9 | GGGAGCGGAAACGAAAACCTATT | 167 |
| Â | CACTGGGCACTACATTCACTCCTG | Â |
ADAMTS-15 | GACACGGCCATCCTCTTCACTCG | 107 |
| Â | AGCAGCTCCTCTTGGGGTCACAC | Â |