Effect of integrin-β3 knockdown on induction of NFATc1 mRNA. CD16- monocytes transfected with either control or integrin-β3 siRNA were cultured with macrophage colony-stimulating factor (M-CSF) (25 ng/ml) + receptor activator of NF-κB ligand (RANKL) (40 ng/ml). Total RNA was extracted 48 hours post-transfection. Semiquantitative RT-PCR analysis was performed using NFATc1-specific and GAPDH-specific primers. Representative results from four independent experiments are shown.