Table 1 Primer sequences
From: Identification of a human peripheral blood monocyte subset that differentiates into osteoclasts
| Molecule | Primer sequence |
|---|---|
| Receptor activator of NF-κB | 5'-TGG CCG CCT AAG TGG AGA TA |
| 3'-TGC GTA GGG ACC ACC TCC TA | |
| c-fms | 5'-GAC GTT TGA GCT CAC CCT TCG ATA |
| 3'-CCT GGT ACT TGG GCT TCT GCT TAT | |
| Tumor necrosis factor receptor-associated factor 6 | 5'-AGA CAA GAC CAT CAA ATC CGG GAG |
| 3'-TCC AGG GCT ATG AAT CAC AAC AGG | |
| c-Fos | 5'-CAG GAG ACA GAC CAA CTA GA |
| 3'-TTC ACG GAC AGA TAA GGT CC | |
| DNAX-activation protein 12 | 5'-ATG GGG GGA CTT GAA CCC |
| 3'-TCA TTT GTA ATA CGG CCT CTG TG | |
| Fc receptor γ chain | 5'-TGA TTC CAG CAG TGG TCT TGC TCT |
| 3'-ATG CAG GCA TAT GTG ATG CCA ACC | |
| Signal regulatory protein β1 | 5'-ACC CAC CTT GGA GGT TAC TCA ACA |
| 3'-TGT AGA TGG CAG AGA CAC CAA CCA | |
| Triggering receptor expressed on myeloid cells 2 | 5'-ATG GAG CCT CTC CGG GTG CT |
| 3'-CTG CGG AAT CTA CAA CCC CA | |
| Osteoclast-associated receptor | 5'-GAG TAG CTG AAA GGA AGA CGC GAT |
| 3'-CAG AGC GCT GAT TGG TCC ATC TTA | |
| Nuclear factor of activated T cells c1 | 5'-TGT GCC GGA ATC CTG AAA CTC AGA |
| 3'-TCC CGT TGC AGA CGT AGA AAC TGA | |
| Integrin αv | 5'-TCC CAT CAG TGG TTT GGA GCA TCT |
| 3'-TCC GAC AGC CAC AGA ATA ACC CAA | |
| Integrin β3 | 5'-TGC CTC AAC AAT GAG GTC ATC CCT |
| 3'-AGA CAC ATT GAC CAC AGA GGC ACT | |
| β-Actin | 5'-GTC CTC TCC CAA GTC CAC ACA |
| 3'-CTG GTC TCA AGT CAG TGT ACA GGT AA | |
| GAPDH | 5'-TGA TGA CAT CAA GAA GGT GGT GAA G |
| 3'-TCC TTG GAG GCC ATG TGG GCC AT |
