Analysis of normal and osteoarthritic canine cartilage mRNA expression by quantitative polymerase chain reaction

Table 1 A list of primer and probe sequences for the genes evaluated

Gene	Forward	Reverse	Probe
ADAMTS5	TGGGTTCCCAAATATGCAG	CTGTCCCATCCGTCACCT	CTGGGAGA
1AGC1	GGGACCTGTGTGAGATCGAC	GTAACAGTGGCCCTGGAACT	AGGAGCTG
BGN	CAGAACAACGACATCTCAGAGC	TCACCAGGACGAGAGCGTA	CTCCACCA
COL1A2	CTATCAATGGTGGTACCCAGTTT	TGTTTTGAGAGGCATGGTTG	GCCTGCTG
COL2A1	CTGGTGAACCTGGACGAGAG	ACCACGATCACCCTTGACTC	CCTCCTGG
COL3A1	GGATGGTGGCTTCCAGTTT	CCAGCTGGACATCGAGGA	GCTGCCTG
COL5A1	AACCTGTCGGATGGCAAGT	CAGTCCAAGATCAAGGTGACAT	CAGCATCC
COL9A3	CGAGGTGCCTCAGGTGAC	ACCCAGCTCTCCTTTGTCC	GAGACCAG
CSPG2	TGGATGGTTTTAATACGTTCAGG	GCCGTAGTCACACGTCTCTG	CTGCCTTC
CTSB	CGGCCTTCACCGTGTACT	GTGACGTGCTGGTACACTCC	CTTCCTGC
CTSD	GGTCCACATGGAGCAGGT	TATGAGGGAGGTGCCTGTGT	TGGGCAGC
DCN	CGCTGTCAGTGCCATCTC	GGGGGAAGATCTTTTGGTACTT	TCCAGTGT
GAPDH	CTGGGGCTCACTTGAAAGG	CAAACATGGGGGCATCAG	CTGCTCCT
Genomic	AACCCTCAAAGATGAGGTTTAGC	ACTCTGGGATCACGCATGT	CTGCCTTC
LUM	ACCTGGAAATTCTTTTAATGTATCATC	CGGTATGTTTTTAAGCTTATTGTAGGA	TGCTGGAG
MMP13	CCGCGACCTTATCTTCATCT	AACCTTCCAGAATGTCATAACCA	AGAGGCAG
RPL13A	CTGCCCCACAAGACCAAG	GGGATCCCATCAAACACCT	CCAGGCTG
SDHA	GGTGGCACTTCTACGACACC	ATGTAGTGGATGGCGTCCTG	CTGGCTGG
TBP	TCCACAGCCTATCCAGAACA	CTGCTGCTGTTGTCTCTGCT	CTGGAGGA
TIMP1	TGCATCCTGCTGTTGCTG	AACTTGGCCCTGATGACG	CCCAGCAG
TIMP2	ATGGGCTGTGAGTGCAAGAT	CACTCATCCGGAGACGAGAT	CTGCCCCA
TIMP4	GCAGAGAGAAAGTCTGAATCATCA	GGCACTGTATAGCAGGTGGTAA	TGTGGCTG
TNC	TGGATGGGACAGTCAAGGA	GCTCAGCTCTGCCAGGTTA	CCACCTCC
VIM	TACAGGAAGCTGCTGGAAGG	CCTCAGGTTCAGGGAAGAAA	GAGCAGGA

ADAMTS5, ADAM metallopeptidase with thrombospondin type 1 motif, 5; AGC1, aggrecan; BGN, biglycan; COL1A2, type I collagen, alpha two chain; COL2A1, type II collagen alpha 1 chain; COL3A1, type III collagen alpha 1 chain; COL5A1, type V collagen alpha 1 chain; COL9A3, type IX collagen alpha 3 chain; CSPG2, chondroitin sulphate proteoglycan 2; CTSB, cathepsin B; CTSD, cathepsin D; DCN, decorin; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; Genomic, Genomic DNA, LUM, lumican;MMP13, matrix metalloproteinase 13; RPL13A, ribosomal protein L13a; SDHA, succinate dehydrogenase complex, subunit A; TBP, TATA box binding protein; TIMP1, tissue inhibitor of metalloproteinase 1; TIMP2, tissue inhibitor of metalloproteinase 2; TIMP4, tissue inhibitor of metalloproteinase 4; TNC, Tenascin C; VIM, vimentin.

ISSN: 1478-6362