Analysis of normal and osteoarthritic canine cartilage mRNA expression by quantitative polymerase chain reaction

Table 3 Change in gene expression, mean 2^-ΔΔCT values, significance and power of comparisons between normal and OA canine articular cartilage

Gene	Number of samples in which expression was detected	2^-ΔΔCT normal	2^-ΔΔCT OA	Fold change in expression (diseased versus normal)	Mann-Whitney U test p value	Power
TIMP4	27	0.109	0.043	-0.608	0.0094	0.859
TIMP2	28	3.959	1.664	-0.580	0.0020	0.844
ADAMTS5	16	0.031	0.019	-0.551	0.8478	0.175
SDHA	28	0.323	0.234	-0.275	0.0476	0.722
VIM	28	32.742	28.909	-0.117	0.5493	0.195
TBP	28	0.106	0.106	0.001	0.8178	0.051
DCN	28	73.034	74.253	0.017	0.5190	0.059
GAPDH	28	1.548	1.648	0.064	0.9633	0.105
RPL13A	28	7.048	7.722	0.096	0.3814	0.275
CTSB	26	0.280	0.476	0.698	0.0060	0.886
AGC	28	0.082	0.155	0.887	0.1670	0.778
TNC	28	2.700	5.205	0.927	0.0099	0.886
BGN	28	15.511	30.984	0.998	0.0043	0.976
CTSD	28	0.148	0.295	0.999	0.0066	0.944
COL9A3	27	0.231	0.546	1.365	0.0304	0.633
TIMP1	28	0.551	1.468	1.663	0.0008	0.853
LUM	28	1.635	4.476	1.738	0.0015	0.991
CSPG2	27	0.079	0.279	2.530	0.0005	0.981
COL5A1	28	0.615	2.188	2.555	0.0069	0.887
COL3A1	26	10.573	37.867	2.581	0.0011	0.982
COL1A2	28	0.805	6.941	7.621	0.0043	0.737
MMP13	26	0.014	0.161	10.322	0.0010	0.857
COL2A1	27	1.412	23.583	15.705	0.0001	0.779

ADAMTS5, ADAM metallopeptidase with thrombospondin type 1 motif, 5; AGC, aggrecan; BGN, biglycan; COL1A2, type I collagen, alpha two chain; COL2A1, type II collagen alpha 1 chain; COL3A1, type III collagen alpha 1 chain; COL5A1, type V collagen alpha 1 chain; COL9A3, type IX collagen alpha 3 chain; CSPG2, chondroitin sulphate proteoglycan 2; CTSB, cathepsin B; CTSD, cathepsin D; DCN, decorin; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; LUM, lumican; MMP13, matrix metalloproteinase 13; OA, osteoarthritis; RPL13A, ribosomal protein L13a; SDHA, succinate dehydrogenase complex, subunit A; TBP, TATA box binding protein; TIMP1, tissue inhibitor of metalloproteinase 1; TIMP2, tissue inhibitor of metalloproteinase 2; TIMP4, tissue inhibitor of metalloproteinase 4; TNC, Tenascin C; VIM, vimentin.

ISSN: 1478-6362