Figure 6

In vitro effect of HGF on IL-4 production by TSK/+ CD4⁺ T cells. CD4⁺ T cells from TSK/+ (H-2^b) mice at the age of 6 weeks (4 × 10⁶ cells/ml/well) were cultured with irradiated (20 Gy) CD11c⁺ DCs from BDF1 (H-2^{b × d}) mice (1 × 10⁶ cells/ml/well) in the presence or absence of human recombinant HGF (10 ng/ml). CD4⁺ T cells from TSK/+ (H-2^b) mice (4 × 10⁶ cells/ml/well) cultured without CD11c⁺ DC stimulation were used as controls. After 72 hours, viable cells (1 × 10⁵ cells/200 μl/well) were harvested and stimulated with anti-CD3 mAb (5 μg/ml) for 48 hours. The concentration of IL-4 in the culture supernatant was measured by ELISA. Data represent the mean ± standard deviation of three independent experiments. *p < 0.05. DC, dendritic cell; HGF, hepatocyte growth factor; T, T cell; TSK/+, tight skin.