Figure 4

Exacerbation of murine models of tumor necrosis factor alpha-related inflammatory diseases in BM-hIL-32 mice. (a) Collagen-antibody-induced arthritis was induced in bone-marrow chimeric human IL-32β mice (BM-hIL-32) (n = 6) and bone-marrow chimeric mice of the mock group (BM-Mock) (n = 4). Mean arthritis scores are shown. (b) Body weight change after induction of trinitrobenzen sulfonic acid (TNBS)-induced colitis in BM-Mock mice (n = 7), in BM-hIL-32 mice (n = 4), and in BM-hIL-32 mice + 200 μg/day intraperitoneal administration of etanercept (n = 4). Control mice (n = 5) were administered only 50% ethanol with PBS. Percentage of initial body weight is shown. (c) Histological scores of TNBS-induced colitis. (d) Relative expression of mouse tumor necrosis factor alpha (mTNFα) in the colon of TNBS-induced colitis mice. *P < 0.05, **P < 0.01, BM-hIL-32 mice versus BM-Mock mice or BM-hIL-32 mice + etanercept.