MLN51 expression in dendritic cells (DCs) and its effect on the proliferation of immature DCs. (a) MLN51 expression is upregulated only in immature bone marrow-derived dendritic cells (BmDCs) in contrast with bone marrow progenitor cells or mature BmDCs. Total RNA was extracted from each sample with Trizol reagent. RT-PCR was performed with mouse MLN51 primers. (b) BC-1 cells (106) were transfected with 4 μg of mMLN51 siRNA (siRNA(+)) or control siRNA (siRNA(-)). The transfected cells were harvested every day and assessed for their proliferation over a period of 4 days. The results are means ± SD obtained from single experiments performed in triplicate cultures. (c) Total RNA was extracted each day from the transfected BC-1 cell cultures. mMLN51 was assessed in each sample by RT-PCR as described previously with primers. Results in (a) and (c) are representative of three separate experiments.