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Figure 3 | Arthritis Research & Therapy

Figure 3

From: The proinflammatory cytokines IL-1β and TNF-α induce the expression of Synoviolin, an E3 ubiquitin ligase, in mouse synovial fibroblasts via the Erk1/2-ETS1 pathway

Figure 3

Induction of Synoviolin (SYN) expression by proinflammatory cytokines. (a) Mouse synovial fibroblasts were starved for 24 hours and then cultivated for 48 hours with each proinflammatory cytokine, including 10 ng/ml of tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, or IL-6. The expression of SYN was tested by Western blotting by using anti-SYN antibody (top panel). As a control, the protein level of actin was detected using the same membrane (bottom panel). (b) The total RNA from the cultured synovial fibroblasts was isolated and reverse-transcribed into cDNA. The levels of SYN cDNA were analyzed by real-time polymerase chain reaction by using SYN-specific primers. Error bars represent the results from three independent experiments (mean ± standard deviation).

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