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Figure 5 | Arthritis Research & Therapy

Figure 5

From: The proinflammatory cytokines IL-1β and TNF-α induce the expression of Synoviolin, an E3 ubiquitin ligase, in mouse synovial fibroblasts via the Erk1/2-ETS1 pathway

Figure 5

ETS1 is involved in interleukin-1β(IL-1β)-induced Synoviolin (SYN) expression. (a) ETS1 or ETS1-DN expression plasmids were transfected into mouse synovial fibroblasts. Cells were then cultured in medium containing 10 ng/ml of IL-1β for 48 hours. The expression of SYN was detected by Western blotting (top panel). The expression of ETS1 and its mutant was detected with anti-ETS1 antibody (middle panel). The same membrane was stripped and reblotted with anti-actin antibody (bottom panel).(b) The expression level of SYN was quantified. Error bars represent three independent experiments (mean ± standard deviation). ETS1-DN, dominant negative mutant of ETS1.

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