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Figure 1 | Arthritis Research & Therapy

Figure 1

From: Identification of novel citrullinated autoantigens of synovium in rheumatoid arthritis using a proteomic approach

Figure 1

Detection of citrullinated synovial autoantigens in rheumatoid arthritis (RA). Proteins extracted from synovium of a patient with RA were separated by two-dimensional electrophoresis, and protein spots were stained by SYPRO Ruby gel stain (a). Then, the proteins were transferred onto nitrocellulose membranes and reacted with (c) anti-modified citrulline (anti-MC) antibodies after modification, (d) pooled serum samples from five patients with RA (diluted at 1:500 per person), and (e) pooled serum samples from five healthy donors (diluted at 1:500 per person). For these experiments, the capability of the anti-MC antibodies to detect citrullinated proteins was confirmed by response to the cell lysate of Escherichia coli treated or not treated with peptidylarginine deiminase (PAD) (b). Protein spots that reacted with the RA sera and the anti-citrulline antibodies, but not with the sera from healthy donors, were thought to be candidates for citrullinated synovial autoantigens, indicated by the numbers 1–30 in (c) and (d).

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