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Figure 3 | Arthritis Research & Therapy

Figure 3

From: Identification of novel citrullinated autoantigens of synovium in rheumatoid arthritis using a proteomic approach

Figure 3

Preparation of recombinant F-actin capping protein α-1 subunit (CapZα-1) with His-tag and citrullination of the recombinant CapZα-1. (a) cDNA for the entire protein coding region of human CapZα-1 was amplified by reverse transcription-polymerase chain reaction and then inserted into the pETBlue-2 vector. The full-length CapZα-1 was produced in Escherichia coli and purified by using histidine-Ni+ affinity (lane 1, produced CapZα-1 before purification; lane 2, CapZα-1 after purification). (b) The recombinant CapZα-1 was reacted with 20 U/mg of peptidylarginine deiminase in various concentrations of CaCl2. Then, the citrulline residues were detected by the anti-modified citrulline (anti-MC) antibodies.

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