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Table 2 Citrullinated autoantigens identified using mass spectrometry

From: Identification of novel citrullinated autoantigens of synovium in rheumatoid arthritis using a proteomic approach

Spot number

Observed

Protein name

Mascot score

Number of peptides

Accession number

Calculated

 

pI

MW (kDa)

    

pI

MW (kDa)

1

5.4

21

n.d.

     

2

5.1

24

n.d.

     

3

5.15

28

n.d.

     

4

5.0

30

n.d.

     

5

5.75

25

n.d.

     

6

6.1

26

n.d.

     

7

5.7

31

Mutant β-actin

101

1

gi| 28336

5.22

42.1

8

5.5

34

n.d.

     

9

5.5

36

n.d.

     

10

5.7

33

n.d.

     

11

5.8

33

Cathepsin D, chain B

120

8

gi| 494296

5.31

26.5

12

6.0

35

n.d.

     

13

5.7

40

n.d.

     

14

5.7

41

n.d.

     

15

5.6

41

F-actin capping protein α-1 subunit (CapZα-1)

85

6

gi| 5453597

5.45

33.1

16

6.25

44

Asporin precursor

Asporin

Periodontal ligament associated protein-1

81

81

81

1

1

1

gi|13625797

gi|41350214

gi|16151085

6.84

5.36

6.43

43.7

43.8

43.9

17

6.4

43

Asporin precursor

36

1

gi| 13625797

6.84

43.7

18

6.25

47

Chain B, crystal structure of fibrinogen fragment D

75

9

gi| 2781208

5.85

37.6

19

6.5

48

Asporin precursor

65

8

gi| 13625797

6.84

43.7

20

5.7

51

Mutant β-actin

48-kDa histamine receptor subunit peptide-4 (internal fragment)

62

62

1

1

gi| 28336

gi| 998467

5.22

4.41

42.1

31.4

21

5.75

58

n.d.

     

22

5.8

61

n.d.

     

23

5.95

61

Protein disulfide-isomerase (EC 5. 3. 4. 1.)

ER60 precursor Glucose-related protein 58 kDa

201

201

20

20

gi|1085373

gi|27805905

6.10

6.23

56.6

56.9

24

6.1

59

Chain A, human mitochondrial aldehyde dehydrogenase complexed with NAD+ and Mn2+

122

2

gi| 6137677

5.70

53.9

25

6.3

58

n.d.

     

26

6.4

67

Human serum albumin in a complex with myristic acid and tri-iodobenzoic acid

Chain A, crystal structure of human serum albumin

83

82

9

9

gi|4389275

gi|3212456

5.69

5.67

66.0

66.4

27

5.85

102

Recombinant human gamma-fibrinogen carboxyl terminal fragment

63

6

gi| 2554640

5.87

28.2

28

5.75

102

Fibrinogen gamma

102

7

gi| 223170

5.54

46.3

29

4.7

62

n.d.

     

30

4.8

62

n.d.

     
  1. MW, molecular weight; n.d., not determined; pI, isoelectric point.