Induction of multiple matrix metalloproteinase (MMP) mRNAs in human dermal fibroblasts (HDF) by Staphylococcus aureus. Confluent monolayers of fibroblasts were exposed to 25 μg/well of total protein from culture supernatants (SUP), 25 μg/well of total protein from bacterial cell lysate, or 10 ng/ml each of rhTNF-α and rhIL-1β for 8 hours. Total RNA was isolated and reverse-transcribed. The cDNA was amplified and quantified using SYBR green real-time polymerase chain reaction. All the MMPs tested were expressed in dermal fibroblasts exposed to S. aureus components. Both culture supernatants and cell lysate from S. aureus significantly enhanced the expression of multiple MMPs (namely, MMP-1, -2, -3, -7, -10, and -11; p < 0.05). The dermal fibroblasts responded similarly to a combination of rhTNF-α and rhIL-1β in terms of multiple MMP mRNA expression. IL, interleukin; TNF, tumor necrosis factor.