Figure 7
From: Abnormal insulin-like growth factor 1 signaling in human osteoarthritic subchondral bone osteoblasts
Syp activation in normal and osteoarthitis (OA) osteoblasts. Cells were grown to confluence and incubated overnight in serum free medium. Cells were then exposed to 50 ng/ml insulin-like growth factor (IGF)-1 for 5 minutes. (a) After cell lysis in RIPA buffer Syp levels were visualized by immunoblotting with an anti-Syp antibody. (b) After cell lysis in RIPA buffer and immunoprecipitation with an anti-phosphotyrosine antibody (PY-20), phosphorylated Syp levels were visualized by immunoblotting with an anti-Syp antibody. Determination of actin was used as the control for loading. Representative data are shown in panel 1 (a,b). Results expressed as arbitrary scanning units are presented as the mean ± SEM (panel 2 (a,b)).