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Figure 1 | Arthritis Research & Therapy

Figure 1

From: The role of synovial macrophages and macrophage-produced cytokines in driving aggrecanases, matrix metalloproteinases, and other destructive and inflammatory responses in osteoarthritis

Figure 1

Fluorescence-activated cell sorting (FACS) analysis of monocyte/macrophage depletion in peripheral blood mononuclear cells (PBMC). (a) The left panel shows FACS analysis of forward scatter (FSC) and side scatter (SSC) of unlabeled PBMC, with the approximate monocyte population indicated with a circle. In the right panels, cells have been incubated with an anti-CD14 phycoerythrin antibody, clearly showing the distinct population of CD14+ cells on FACS (upper panel) and in a histogram (lower panel). (b) The corresponding panels show monocyte-depleted PBMC. In the left panel, the monocyte population is reduced, although the debris and cell clusters blur this effect. In the right upper panel, the CD14+ population virtually disappears, as confirmed by the histogram in the right lower panel. (c) The corresponding panels show the enriched monocytes/macrophages. In the left panel, although there is still a fair amount of debris and cell clusters, the monocyte population is considerably enriched. In the right upper panel, the CD14+ population is also very much enriched, as confirmed by the histogram in the panel below. (d) A functional assay using enzyme-linked immunosorbent assay analysis of spontaneous and lipopolysaccharide-stimulated (open bars) tumour necrosis factor-alpha (TNF-α) production in monocyte/macrophage-depleted and -undepleted PBMCs verifies that the monocyte/macrophage depletion is effective.

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