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Table 1 Oligonucleotide primers used for reverse transcription-polymerase chain reaction

From: The role of synovial macrophages and macrophage-produced cytokines in driving aggrecanases, matrix metalloproteinases, and other destructive and inflammatory responses in osteoarthritis

Target template Polymerase chain reaction primers Product size (bp) Annealing temperature (°C)
GAPDH 5'-TGGTATCGTGGAAGGACTCAT
5'-GTGGGTGTCGCTGTTGAAGTC
370 53
ADAMTS4 5'-GTCTGTGTCCAGGGCCGATGC
5'-GCCGCCGAAGGATCTCCAGAA
541 61.8
ADAMTS5 5'-GCGGATGTGTGCAAGCTGACC
5'-AGTAGCCCATGCCATGCAGGA
487 57.4
MMP-1 5'-ACAAATCCCTTCTACCCGGAA
5'-GGATCCATAGATCGTTTATAT
314 50.8
MMP-3 5'-CTTTTGGCGAAAATCTCTCAG
5'-AAAGAAACCCAAATGCTTCAA
404 50
MMP-9 5'-GGCCTCCAACCACCACCACA
5'-CGCCCAGAGAAGAAGAAAAGC
400 61
MMP-13 5'-TTCTGGCACACGCTTTTCCTC
5'-GGTTGGGGTCTTCATCTCCTG
273 53
TIMP-1 5'-CCACCTTATACCAGCGTTAT
5'-CCTCACAGCCAACAGTGTAGG
282 54
TIMP-2 5'-GTGGACTCTGGAAACGACAT
5'-TCTTCTTCTGGGTGGTGCTCA
265 54
TIMP-3 5'-GGGAAGAAGCTGGTAAAGGAG
5'-GCCGGATGCAGGCGTAGTGTT
418 54
Link protein 5'-GGAAGCAGAGCAAGCCAAGGT
5'-GAAGGAGGCGATCACAGCATC
448 56.1
  1. Primer sequences correspond to sequences for human cDNAs deposited to GenBank. Where a mixed base is indicated (that is, for GAPDH), the sequence also corresponds to the analogous rat cDNA. ADAMTS, a disintegrin and metalloprotease with thrombospondin motifs; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; MMP, matrix metalloproteinase; TIMP, tissue inhibitor of matrix metalloproteinase.