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Table 1 Oligonucleotide primers used for reverse transcription-polymerase chain reaction

From: The role of synovial macrophages and macrophage-produced cytokines in driving aggrecanases, matrix metalloproteinases, and other destructive and inflammatory responses in osteoarthritis

Target template

Polymerase chain reaction primers

Product size (bp)

Annealing temperature (°C)

GAPDH

5'-TGGTATCGTGGAAGGACTCAT

5'-GTGGGTGTCGCTGTTGAAGTC

370

53

ADAMTS4

5'-GTCTGTGTCCAGGGCCGATGC

5'-GCCGCCGAAGGATCTCCAGAA

541

61.8

ADAMTS5

5'-GCGGATGTGTGCAAGCTGACC

5'-AGTAGCCCATGCCATGCAGGA

487

57.4

MMP-1

5'-ACAAATCCCTTCTACCCGGAA

5'-GGATCCATAGATCGTTTATAT

314

50.8

MMP-3

5'-CTTTTGGCGAAAATCTCTCAG

5'-AAAGAAACCCAAATGCTTCAA

404

50

MMP-9

5'-GGCCTCCAACCACCACCACA

5'-CGCCCAGAGAAGAAGAAAAGC

400

61

MMP-13

5'-TTCTGGCACACGCTTTTCCTC

5'-GGTTGGGGTCTTCATCTCCTG

273

53

TIMP-1

5'-CCACCTTATACCAGCGTTAT

5'-CCTCACAGCCAACAGTGTAGG

282

54

TIMP-2

5'-GTGGACTCTGGAAACGACAT

5'-TCTTCTTCTGGGTGGTGCTCA

265

54

TIMP-3

5'-GGGAAGAAGCTGGTAAAGGAG

5'-GCCGGATGCAGGCGTAGTGTT

418

54

Link protein

5'-GGAAGCAGAGCAAGCCAAGGT

5'-GAAGGAGGCGATCACAGCATC

448

56.1

  1. Primer sequences correspond to sequences for human cDNAs deposited to GenBank. Where a mixed base is indicated (that is, for GAPDH), the sequence also corresponds to the analogous rat cDNA. ADAMTS, a disintegrin and metalloprotease with thrombospondin motifs; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; MMP, matrix metalloproteinase; TIMP, tissue inhibitor of matrix metalloproteinase.