Target gene | Primer sequence (5'-3') | PCR product size (bp) | Annealing temperature (°C) | Number of PCR cycles | GenBank accession number |
---|---|---|---|---|---|
ALP | Â | Â | Â | Â | Â |
Sense | ccaacgtggctaagaatgTC | 434 | 58 | 30 | NM_000478 |
Antisense | catctcgttgtctgagtacc | Â | Â | Â | Â |
OCN | Â | Â | Â | Â | Â |
Sense | ggtgcagcctttgtgtccaagc | 159 | 62 | 29 | NM_199173 |
Antisense | GTCAGCCAACTCGTCACAGTCC | Â | Â | Â | Â |
OPN | Â | Â | Â | Â | Â |
Sense | AGCCGTGGGAAGGACAGTTATG | 472 | 62 | 29 | NM_000582 |
Antisense | GAGTTTCCATGAAGCCACAAAC | Â | Â | Â | Â |
IGF-I | Â | Â | Â | Â | Â |
Sense | GAGCCTGCGCAATGGAATAAAG | 344 | 62 | 33 | NM_000618 |
Antisense | CCTGTCTCCACACACGAACTG | Â | Â | Â | Â |
IGF-II | Â | Â | Â | Â | Â |
Sense | GAGGAGTGCTGTTTCCGCAG | 263 | 62 | 27 | NM_000612 |
Antisense | ACGTTTGGCCTCCCTGAACG | Â | Â | Â | Â |
TGF-β1 [53] |  |  |  |  |  |
Sense | CTAGACCCTTTCTCCTCCAGGAGACG | 224 | 62 | 26 | NM_000660 |
Antisense | GCTGGGGGTCTCCCGGCAAAAGGT | Â | Â | Â | Â |
COL1A1 | Â | Â | Â | Â | Â |
Sense | CGGCAAGGTGTTGTGCGATG | 339 | 62 | 33 | NM_000088 |
Antisense | CACGGAAATTCCTCCGGTTG | Â | Â | Â | Â |
COL1A2 | Â | Â | Â | Â | Â |
Sense | CGCTGGTGAAGTTGGCAAACCA | 778 | 66 | 31 | NM_000089 |
Antisense | GAGGACCACGAAGCCCTTCTTTC | Â | Â | Â | Â |
GAPDH [16] | Â | Â | Â | Â | Â |
Sense | CATGGAGAAGGCTGGGGCTC | 415 | 62 | 23 | NM_002046 |
Antisense | CACTGACACGTTGGCAGTGG | Â | Â | Â | Â |