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Table 1 Primer design for semi-quantitative reverse transcription-polymerase chain reaction analysis

From: Differential gene expression of bone anabolic factors and trabecular bone architectural changes in the proximal femoral shaft of primary hip osteoarthritis patients

Target gene

Primer sequence (5'-3')

PCR product size (bp)

Annealing temperature (°C)

Number of PCR cycles

GenBank accession number

ALP

     

Sense

ccaacgtggctaagaatgTC

434

58

30

NM_000478

Antisense

catctcgttgtctgagtacc

    

OCN

     

Sense

ggtgcagcctttgtgtccaagc

159

62

29

NM_199173

Antisense

GTCAGCCAACTCGTCACAGTCC

    

OPN

     

Sense

AGCCGTGGGAAGGACAGTTATG

472

62

29

NM_000582

Antisense

GAGTTTCCATGAAGCCACAAAC

    

IGF-I

     

Sense

GAGCCTGCGCAATGGAATAAAG

344

62

33

NM_000618

Antisense

CCTGTCTCCACACACGAACTG

    

IGF-II

     

Sense

GAGGAGTGCTGTTTCCGCAG

263

62

27

NM_000612

Antisense

ACGTTTGGCCTCCCTGAACG

    

TGF-β1 [53]

     

Sense

CTAGACCCTTTCTCCTCCAGGAGACG

224

62

26

NM_000660

Antisense

GCTGGGGGTCTCCCGGCAAAAGGT

    

COL1A1

     

Sense

CGGCAAGGTGTTGTGCGATG

339

62

33

NM_000088

Antisense

CACGGAAATTCCTCCGGTTG

    

COL1A2

     

Sense

CGCTGGTGAAGTTGGCAAACCA

778

66

31

NM_000089

Antisense

GAGGACCACGAAGCCCTTCTTTC

    

GAPDH [16]

     

Sense

CATGGAGAAGGCTGGGGCTC

415

62

23

NM_002046

Antisense

CACTGACACGTTGGCAGTGG

    
  1. ALP, alkaline phosphatase; COL1A, collagen type I alpha chain; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; IGF, insulin-like growth factor; OCN, osteocalcin; OPN, osteopontin; PCR, polymerase chain reaction; TGF-β1, transforming growth factor-β1.