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Table 1 Microscopic variables for fibroblast-like synovial cells

From: Effects of hyaluronan treatment on lipopolysaccharide-challenged fibroblast-like synovial cells

 

Day 1 (pre-lipopolysaccharide challenge)

Day 1 (post-lipopolysaccharide challenge)

Day 2

 

Group 1

Group 2

Group 3

Group 4

Group 1

Group 2

Group 3

Group 4

Group 1

Group 2

Group 3

Group 4

Cellular morphology (median and range)

0 (0-0)

0 (0-0)

0 (0-0)

0 (0-0)

0 (0-0)

3a (0–4)

4a (3–4)

1 (0–4)

0 (0-0)

3b (0–4)

4b (3–4)

0 (0–4)

Cell count (104) (mean ± SEM)

20

20

20

20

-

-

-

-

118c ± 33

46 ± 3

32 ± 1

49 ± 7

Cell viability (%) (mean ± SEM)

-

-

-

-

-

-

-

-

97 ± 0.3

96 ± 1.2

94 ± 2.6

96 ± 0.1

  1. Triplicate samples were performed for each of the three individual donors in the four groups. Group 1, unchallenged control; group 2, lipopolysaccharide control; group 3, pretreatment and sustained treatment with lower molecular weight hyaluronan product; group 4, pretreatment and sustained treatment with higher molecular weight hyaluronan product. 0, >95% attached; 1, 5–25% detached; 2, 26–50% detached; 3, 51–75% detached; 4, >76% detached. SEM, standard error of the mean; -, values not determined. a,b,cSignificant difference exists (P < 0.05).