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Table 3 Comparison of the fold changes between species-specific microarray analysis and real-time RT-PCR performed on fibroblast-like synovial cells exposed to lipopolysaccharide

From: Effects of hyaluronan treatment on lipopolysaccharide-challenged fibroblast-like synovial cells

 

Fold change after 100 ng/ml lipopolysaccharide challenge

Fold change after 1,000 ng/ml lipopolysaccharide challenge

Real-time RT-PCR primer

 

Microarray analysis

Real-time RT-PCR

Microarray analysis

Real-time RT-PCR

 

IL-1α

16

155

34

290

Forward, TTGTGCCAACCAATGAGATCA

     

Reverse, TTCATGCTTTGCCTTCTTCTTG

TNFα

5

6

6

10

Forward, GACTTGAAGTTTTCTAAGCGATGCT

     

Reverse, GGATCCACTGCCACGTACTTG

Prostaglandin peroxide synthase

3

2

3

4

Forward, GGCCAGTTTTCCTCACCAAA

     

Reverse, AAATAAAGCTCTCTGCTTTTCATGAA

  1. The fold changes are normalized to unchallenged fibroblast-like synovial cells.
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Arthritis Research & Therapy

ISSN: 1478-6362

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