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Figure 2 | Arthritis Research & Therapy

Figure 2

From: Effect of cyclooxygenase inhibition on cholesterol efflux proteins and atheromatous foam cell transformation in THP-1 human macrophages: a possible mechanism for increased cardiovascular risk

Figure 2

Quantitation of ABCA1 message in THP-1 cells exposed to NS398. Dose-dependent decrease in ABCA1 mRNA expression in THP-1 monocytes treated with the COX-2 inhibitor NS398. Cultured THP-1 monocytic cells were untreated or exposed to NS398 for 18 hours. After isolation of total RNA, the RNA was reverse-transcribed and the cDNA amplified by quantitative real-time polymerase chain reaction as described. Signals obtained from the amplification of GAPDH message were used as internal controls. At 100 mM NS398 concentration, cell death was statistically significant (14.8% ± 6.3%). ABCA1, ATP-binding cassette transporter A1; COX, cyclooxygenase; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; NS398, N-(2-cyclohexyloxy-4-nitrophenyl)methanesulfonamide. ** p < 0.01.

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Arthritis Research & Therapy

ISSN: 1478-6362

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