Decrease in ABCA1 protein in THP-1 macrophages exposed to NS398. Cultured THP-1 human macrophages were untreated or exposed to increasing concentrations of NS398 for 18 hours. Total cell protein was isolated and ABCA1 detected with specific rabbit polyclonal anti-ABCA1 antibody. Western blotting was performed with an anti-β-actin antibody to confirm equal protein loading. ABCA1, ATP-binding cassette transporter A1; NS398, N-(2-cyclohexyloxy-4-nitrophenyl)methanesulfonamide.