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Figure 3 | Arthritis Research & Therapy

Figure 3

From: The rheumatoid arthritis shared epitope increases cellular susceptibility to oxidative stress by antagonizing an adenosine-mediated anti-oxidative pathway

Figure 3

The shared epitope (SE) attenuates adenosine-induced protection against oxidative DNA damage. (a) The potency of adenosine-mediated anti-oxidative signaling was determined in L-cell transfectants expressing cDNA corresponding to DRB1*0401 (L565.5), DRB1*0402 (L514.3), or DRB1*0403 (L259.3). (b) Human M1 fibroblasts were incubated for 1 hour with or without 24 μg/ml of SE-positive (T-DRB1*0401) or SE-negative (T-DRB1*0401) tetrameric molecules. At the end of incubation, the extent of adenosine-induced protection against oxidative DNA damage was determined as above. (c) M1 human fibroblasts were pre-incubated overnight in the absence or presence of 2.5 μg/ml of either HBc*0401 or HBc*0402 multimeric proteins. At the end of incubation, the extent of adenosine-induced protection against oxidative DNA damage was determined. *p < 0.05.

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