Figure 2

Cleavage of the PM/Scl-75 protein in various cell types treated with different apoptosis inducers. CCRF-CEM cells were treated with actinomycin D (A), anisomycin (AN), cycloheximide (C), staurosporin (S) or anti-Fas mAb 7C11 (α-Fas); Peer cells were treated with actinomycin D (A), anisomycin (AN), cycloheximide (C) or staurosporin (S). Cell extracts were analyzed by 10% SDS-PAGE and Western blotting with the anti-PM/Scl-75 rabbit serum. Extracts from mock-treated cells were loaded in lanes 1 and 7. The positions of molecular mass markers are indicated in kDa at the right and the positions of the relevant polypeptides are shown at the left. Filled arrowheads indicate full-length proteins and open arrowheads indicate cleavage products; the asterisk indicates a cross-reactivity with an unidentified protein.