Figure 3
From: Caspase-mediated cleavage of the exosome subunit PM/Scl-75 during apoptosis
The apoptotic cleavage product of PM/Scl-75 remains associated with the exosome. Immunoprecipitations from control (0 hours) and apoptotic (8 hours incubated with anti-Fas antibody) Jurkat cell extracts were performed with normal rabbit serum (lanes 3 and 4), anti-PM/Scl-75 (lanes 5 and 6) and anti-hRrp46p (lanes 7 and 8) rabbit sera, and patient serum Ven96 (lanes 9 and 10). In lanes 1 and 2 input samples, 20% of the total control and apoptotic cell extracts, respectively, were loaded. Immunoprecipitates were analyzed by 10% SDS-PAGE and Western blotting with a polyclonal anti-PM/Scl-75 mouse serum. The positions of the PM/Scl-75 protein (filled arrowhead), its cleavage product (open arrowhead) and a cross-reactivity of the anti-PM/scl-75 serum (asterisk) are indicated at the left. The positions of molecular mass markers are indicated in kDa at the right.