Effects of exogenous galectin-3 (gal-3) on markers of subchondral bone osteoblasts. Osteoblasts were treated with 1 or 10 μg/ml of recombinant human gal-3 in the presence or absence of vitamin D3. Both (a) alkaline phosphatase and (b) osteocalcin expression were analyzed by real time RT-PCR. Insert illustrates the protein level of osteocalcin. N = 4.