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Figure 1 | Arthritis Research & Therapy

Figure 1

From: Tumor necrosis factor alpha and adalimumab differentially regulate CD36 expression in human monocytes

Figure 1

Regulation of CD36 membrane expression by tumor necrosis factor (TNF)α and adalimumab (Ada). (a) TNFα reduces the membrane expression of CD36: time course. Human monocytes were incubated with macrophage-serum-free medium (M-SFM) alone (control), or with M-SFM containing TNFα (10 ng/ml) for 6 to 12 and 24 h. Membrane expression of CD36 was quantified using flow cytometry. Data represent the geometric mean ± standard error (SE) of the fluorescence measured in three experiments in duplicate. *Significantly different from control (p < 0.05). (b) TNFα reduces membrane expression of CD36: dose effect. Human monocytes were incubated for 24 h with M-SFM alone (control), or with M-SFM containing TNFα at increasing concentrations (0.1, 1, or 10 ng/ml) for 24 h. Membrane expression of CD36 was quantified using flow cytometry. Data represent the geometric mean ± SE of the fluorescence measured in four experiments. *Significantly different from control (p < 0.05). (c) The reduction of membrane expression of CD36 induced by TNFα is inhibited by adalimumab independently of TNFα. Human monocytes were incubated with M-SFM alone (control), or with M-SFM containing either TNFα alone (10 ng/ml), TNFα combined with adalimumab (Ada; 1 μg/ml) or adalimumab alone for 24 h. Membrane expression of CD36 was quantified using flow cytometry. Data represent the geometric mean ± SE of the fluorescence measured in four experiments. *Significantly different from control (p < 0.05). (d) The increase in CD36 membrane expression induced by adalimumab is antibody-specific. Human monocytes were incubated with M-SFM alone (control), or with M-SFM containing either adalimumab (Ada; 1 μg/ml), or rituximab (Ritux; 1 μg/ml), a human anti-CD20 IgG1 monoclonal antibody, as a control antibody for 24 h. Membrane expression of CD36 was quantified using flow cytometry. Data represent the geometric mean ± SE of the fluorescence measured in four experiments in duplicate. *Significantly different from control (p < 0.05). (e) Adalimumab increases membrane expression of CD36: time course. Human monocytes were incubated with M-SFM alone (control), or with M-SFM containing adalimumab (Ada; 1 μg/ml) for 6 to 12 and 24 h. Membrane expression of CD36 was quantified using flow cytometry. Data represent the geometric mean ± SE of the fluorescence measured in three experiments in duplicate. *Significantly different from control (p < 0.05).

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