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Table 1 Expression of extranuclear HMGB1 compared to those of TNF and IL-1β

From: Morphological characterization of intra-articular HMGB1 expression during the course of collagen-induced arthritis

   Lining Sublining Destructive zone Cartilage Vessels
Days p.i. M.A.I. HMGB1 TNF IL-1β HMGB1 TNF IL-1β HMGB1 TNF IL-1β HMGB1 TNF IL-1β HMGB1 TNF IL-1β
   1    1    1    1    1   
0 0 0 0.5 0.5 0 0 0 - - - 1 0 1 0 0 0
3 0 0 0.5 0.5 0 0 0 - - - 1 0 1 0 0 0
6 0 0 0.5 0.5 0 0 0 - - - 1 0 1 0 0 0
10 0 0.5 0.5 0.5 0.5 0 0 - - - 1 0 1 0 0 0
15 3.2 ± 1.8 1 1 1 1 0.5 0 - - - 1 0 1 0 0.5 0
21 9.8 ± 3.3 2 4 2 3 3 3 3 3 3 2 0 2 1 3 1
28 11.0 ± 2.3 2 4 2 3 3 3 3 3 3 2 0 2 1 3 1
38 11.8 ± 2.6 2 4 2 3 3 3 3 3 3 2 0 2 1 3 1
  1. The relative frequencies of positively stained cells in the articular tissue were estimated by immunohistochemistry and assigned an expression score on a scale of 0 to 4: 0, negative cells; 0.5, less than 0.5%; 1, 0.5% to 5%; 2, 5% to 20%; 3, 20% to 50%; and 4, more than 50% positively stained cells. For clinical evaluation, a mean arthritis index (M.A.I.) was calculated for the group of four animals studied per time point and expressed as the mean ± standard deviation. The destructive zone did not appear until day 21 p.i. and was defined as synovial tissue adjacent to areas of cartilage and bone destruction. HMGB1, high-mobility group chromosomal box protein 1; IL-1β, interleukin-1-beta; p.i., post-immunization; TNF, tumor necrosis factor.