Morphological characterization of intra-articular HMGB1 expression during the course of collagen-induced arthritis

Table 2 HMGB1 expression at a protein level compared to HMGB1 mRNA expression

		Lining		Sublining		Destructive zone		Cartilage		Vessels
Days p.i.	M.A.I.	HMGB1 protein	HMGB1 mRNA	HMGB1 protein	HMGB1 mRNA	HMGB1 protein	HMGB1 mRNA	HMGB1 protein	HMGB1 mRNA	HMGB1 protein	HMGB1 mRNA
0	0	0	0.5	0	0.5	-	-	1	0.5	0	0.5
3	0	0	0.5	0	0.5	-	-	1	0.5	0	0.5
6	0	0	0.5	0	0.5	-	-	1	0.5	0	0.5
10	0	0.5	0.5	0.5	0.5	-	-	1	0.5	0	0.5
15	3.2 ± 1.8	1	0.5	1	0.5	-	-	1	0.5	0	0.5
21	9.8 ± 3.3	2	1	3	1	3	4	2	1	1	1
28	11.0 ± 2.3	2	1	3	1	3	4	2	1	1	1
38	11.8 ± 2.6	2	1	3	1	3	4	2	1	1	1

Most cells expressed a low HMGB1 mRNA labeling at all time points. To be able to compare the relative frequencies of cells with HMGB1 protein expression to those of cells with upregulated HMGB1 mRNA expression, an estimation of expression scores similar to that used in Table 1 was used for in situ results; cells with numerous grains were regarded as positive. HMGB1, high-mobility group chromosomal box protein 1; M.A.I., mean arthritis index; p.i., post-immunization.

ISSN: 1478-6362