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Table 3 Absorption of CSF autoantibodies to various components of ribosomal P proteins by neuronal cells

From: Association of cerebrospinal fluid anti-ribosomal P protein antibodies with diffuse psychiatric/neuropsychological syndromes in systemic lupus erythematosus

Patient Autoantibodies Without absorption With absorption
1 Anti-whole P (μg/ml) 7.243 1.435
  Anti-PC22 (μg/ml) 3.456 1.019
  Anti-PEX.C22 (μg/ml) 3.787 0.416
  Anti-N (U/ml) 4.083 1.950
2 Anti-whole P (μg/ml) 0.140 0.050
  Anti-PC22 (μg/ml) 0.070 0.042
  Anti-PEX.C22 (μg/ml) 0.070 0.008
  Anti-N (U/ml) 0.789 0.588
  1. Cerebrospinal fluid (CSF) samples (50 μl/well) were incubated in wells of a 96-well flat-bottomed microtiter plate with or without confluent SK-N-MC cells fixed with 1% paraformaldehyde at room temperature for 2 hours. After the incubation, CSF samples were recovered and were examined for anti-whole P, anti-PC22, anti-PEX.C22, and anti-N as described in Materials and methods. Anti-N, anti-neuronal cell antibodies; anti-PC22, antibodies directed against the C-terminal 22-amino acid sequences of ribosomal P protein; anti-PEX.C22, autoantibodies directed against the ribosomal P protein epitopes other than the C-terminal 22-amino acid sequence; anti-whole P, antibodies to the whole ribosomal P proteins.