Figure 2From: Accelerated cellular senescence in degenerate intervertebral discs: a possible role in the pathogenesis of intervertebral disc degenerationSenescence biomarker immunohistochemistry. (a) p16INK4a immunopositivity in the nucleus pulposus of human intervertebral discs. (b) Immunoglobulin G controls were negative. (c) Senescence-associated β-galactosidase staining in directly extracted cells from non-degenerate discs. (d) Senescence-associated β-galactosidase staining in directly extracted cells from degenerate discs (positive cells indicated with arrows). Scale bars = 190 μm (a, b) and 370 μm (c, d).Back to article page