Accelerated cellular senescence in degenerate intervertebral discs: a possible role in the pathogenesis of intervertebral disc degeneration

Table 1 Intervertebral disc samples used for telomere length assay, senescence-associated β-galactosidase staining, and p16^INK4Aimmunohistochemistry

Laboratory number	Gender	Age (years)	Cell type	Disc level	Cell source	Histological grade
1	M	37	AF	L4/5	PM	1
2	M	37	AF	L5/S1	PM	1
3^a	M	37	NP	L4/5	PM	1
4^a	M	37	NP	L5/S1	PM	1
5	M	47	AF	L2/3	PM	1
6	M	47	AF	L3/4	PM	1
7^a	M	47	NP	L2/3	PM	1
8	M	47	NP	L3/4	PM	1
9	M	47	NP	L5/S1	PM	1
10	M	47	AF	L4/5	PM	2
11	M	47	NP	L4/5	PM	2
12	M	59	NP	L4/5	PM	2
13	M	59	AF	L4/5	PM	2
14	M	62	AF	L3/4	PM	2
15	M	62	AF	L4/5	PM	2
16^a	M	62	NP	L3/4	PM	2
17	M	62	NP	L4/5	PM	2
18	M	37	AF	L1/2	PM	3
19	M	37	NP	L1/2	PM	3
20	M	74	AF	L3/4	PM	3
21	M	37	AF	L2/3	PM	4
22	M	37	AF	L3/4	PM	4
23	M	37	NP	L2/3	PM	4
24	M	37	NP	L3/4	PM	4
25^a	F	49	NP	L4/5	Surgical	4
26	M	44	NP	L4/5	Surgical	5
27	M	62	AF	L5/S1	PM	5
28	M	62	NP	L5/S1	PM	5
29	M	74	AF	L4/5	PM	5
30	M	74	AF	L2/3	PM	6
31^a	F	49	NP	L5/S1	Surgical	8
32	F	15	Tissue	L4/5	Surgical	0
33	F	27	Tissue	L5/S1	Surgical	0
34	M	39	Tissue	L4/5	Surgical	0
35	F	44	Tissue	L4/5	Surgical	0
36	F	20	Tissue	L5/S1	Surgical	2
37	M	40	Tissue	L4/5	Surgical	2
38	M	47	Tissue	L4/5	Surgical	2
39	F	27	Tissue	L4/5	Surgical	3
40	M	31	Tissue	L4/5	Surgical	3
41	F	57	Tissue	L4/5	Surgical	3
42	M	59	Tissue	L5/S1	Surgical	3
43	M	28	Tissue	L4/5	Surgical	5
44	F	34	Tissue	L3/4	Surgical	5
45	M	39	Tissue	L5/S1	Surgical	5
46	M	55	Tissue	L3/4	Surgical	5
47	F	27	Tissue	L4/5	Surgical	7
48	F	56	Tissue	L5/S1	Surgical	7
49	M	33	Tissue	L5/S1	Surgical	8
50	F	40	Tissue	L4/5	Surgical	8
51	M	54	Tissue	L4/5	Surgical	8
52	M	32	Tissue	L4/5	Surgical	10
53	F	41	Tissue	L5/S1	Surgical	12

Intervertebral disc samples 1 to 31 were used for telomere length assay, and samples 32 to 53 were used for p16^INK4aimmunohistochemistry. ^aIntervertebral disc samples used for senescence-associated β-galactosidase staining. AF, annulus fibrosus; F, female; M, male; NP, nucleus pulposus; PM, postmortem tissue.

ISSN: 1478-6362