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Table 2 Polymerase chain reaction primer and probe sequences, amplicon sizes, and efficiencies

From: Accelerated cellular senescence in degenerate intervertebral discs: a possible role in the pathogenesis of intervertebral disc degeneration

Standard polymerase chain reaction conditions

Target

Forward primer

Reverse primer

Amplicon size

GAPDH

5' CCC ATC ACC ATC TTC CAG G 3'

5' GGC CAT CCA CAG TCT TCT G 3'

354 bp

hTERT

5' GCC TGA GCT GTA CTT TGT CAA 3'

5' AGG CTG CAG AGC AGC GTG GAG AGG 3'

422 bp

Real-time polymerase chain reaction primers and probes

Target

Forward primer

Probe

Reverse primer

Efficiency

18s

PDAR

PDAR

PDAR

99.65%

P16INK4a

5' GGC TCT ACA CAA GCT TCC TTT CC 3'

5' 6 FAM – CCC CCA CCC TGG CTC TGA CCA – TAMRA

5' TCA TGA CCT GCC AGA GAG AAC A 3'

99.22%

MMP-13

5' CCC CAG GCA TCA CCA TTC AAG 3'

5' 6 FAM – AGG GGT CCT GGC TGC CTT CCT CTT C – TAMRA 3'

5' GAC AAA TCA TCT TCA TCA CCA CCA C 3'

99.77%

ADAMTS 5

5' GGA CCT ACC ACG AAA GCA GAT C 3'

5' 6 FAM – CCC AGG ACA GAC CTA CGA TGC CAC C – TAMRA 3'

5' GCC GGG ACA CAC GGA GTA 3'

99.74%

  1. ADAMTS 5, a disintegrin and metalloprotease with thrombospondin motifs 5; bp, base pairs; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; hTERT, human telomerase reverse transcriptase; MMP-13, matrix metalloproteinase-13; PDAR, pre-developed assay reagent.