Standard polymerase chain reaction conditions | ||||
---|---|---|---|---|
Target | Forward primer | Reverse primer | Amplicon size | |
GAPDH | 5' CCC ATC ACC ATC TTC CAG G 3' | 5' GGC CAT CCA CAG TCT TCT G 3' | 354 bp | |
hTERT | 5' GCC TGA GCT GTA CTT TGT CAA 3' | 5' AGG CTG CAG AGC AGC GTG GAG AGG 3' | 422 bp | |
Real-time polymerase chain reaction primers and probes | ||||
Target | Forward primer | Probe | Reverse primer | Efficiency |
18s | PDAR | PDAR | PDAR | 99.65% |
P16INK4a | 5' GGC TCT ACA CAA GCT TCC TTT CC 3' | 5' 6 FAM – CCC CCA CCC TGG CTC TGA CCA – TAMRA | 5' TCA TGA CCT GCC AGA GAG AAC A 3' | 99.22% |
MMP-13 | 5' CCC CAG GCA TCA CCA TTC AAG 3' | 5' 6 FAM – AGG GGT CCT GGC TGC CTT CCT CTT C – TAMRA 3' | 5' GAC AAA TCA TCT TCA TCA CCA CCA C 3' | 99.77% |
ADAMTS 5 | 5' GGA CCT ACC ACG AAA GCA GAT C 3' | 5' 6 FAM – CCC AGG ACA GAC CTA CGA TGC CAC C – TAMRA 3' | 5' GCC GGG ACA CAC GGA GTA 3' | 99.74% |