Figure 3

Humoral immune responses in DBA mice immunized with type II collagen (CII) and complete Freund's adjuvant (CFA) and treated with 1-methyl-tryptophan (1-MT) or vehicle. (a) Concentrations of antibodies (total concentrations of IgGAM [immunoglobulins G, A, and M]) to heterologous (human) CII were determined in the serum of DBA/1 mice immunized with CII and CFA and treated with 1-MT or vehicle. (b) Concentrations of antibodies (total concentrations of IgGAM) to autologous (mouse) CII were determined in the serum of DBA/1 mice immunized with CII and CFA and treated with 1-MT or vehicle. Sera were obtained on days 5, 22, 33, and 54. Values are presented as the mean and standard error of the mean of 25 1-MT-treated and 20 vehicle-treated DBA/1 mice per group and represent two independent experiments. *p < 0.05 between vehicle and 1-MT-treated mice on the corresponding days.