Table 2 Primer and product sizes of real-time PCR validated genes
Gene product | Forward primer (5'→3') | Reverse primer (5'→3') | Size (bp) | TA/tamp |
|---|---|---|---|---|
Aldolase | TCATCCTCTTCCATgAGACACTCTA | ATTCTgCTggCAgATACTggCATAA | 314 | 58°C/30 s |
TGF-β1 | gTTCAAgCAgAGTACACACAgC | gTATTTCTggTACAgCTCCACg | 157 | 60°C/20 s |
TGF-β2 | ATgCggCCTATTgCTTTAgA | TAAgCTCAggACCCTgCTgT | 185 | 60°C/20 s |
TGF-β3 | CAgggAgAAAATCCAggTCA | CCTggAAggCgTCTAACCAAg | 179 | 58°C/20 s |
THBS1 | gATCCTggACTCgCTgTAgg | CCgAgTATCCCTgAgCCCTC | 202 | 60°C/20 s |
TGFBR1 | ATCACCTggCCTTggTCCTgTgg | GgTCCTCTTCATTTggCACTCgATg | 140 | 54°C/20 s |
SkiL | CAgTggAAACTgATggAgAgC | ggAAgAggCAgAAATACAgTAgg | 193 | 55°C/20 s |
MMP-11 | ggTgTACgACggTgAAAAgCC | CAgggTCAAACTTCCAgTAgAgg | 353 | 64°C/30 s |