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Table 2 Primer and product sizes of real-time PCR validated genes

From: Constitutive upregulation of the transforming growth factor-β pathway in rheumatoid arthritis synovial fibroblasts

Gene product Forward primer (5'→3') Reverse primer (5'→3') Size (bp) TA/tamp
Aldolase TCATCCTCTTCCATgAGACACTCTA ATTCTgCTggCAgATACTggCATAA 314 58°C/30 s
TGF-β1 gTTCAAgCAgAGTACACACAgC gTATTTCTggTACAgCTCCACg 157 60°C/20 s
TGF-β2 ATgCggCCTATTgCTTTAgA TAAgCTCAggACCCTgCTgT 185 60°C/20 s
TGF-β3 CAgggAgAAAATCCAggTCA CCTggAAggCgTCTAACCAAg 179 58°C/20 s
THBS1 gATCCTggACTCgCTgTAgg CCgAgTATCCCTgAgCCCTC 202 60°C/20 s
TGFBR1 ATCACCTggCCTTggTCCTgTgg GgTCCTCTTCATTTggCACTCgATg 140 54°C/20 s
SkiL CAgTggAAACTgATggAgAgC ggAAgAggCAgAAATACAgTAgg 193 55°C/20 s
MMP-11 ggTgTACgACggTgAAAAgCC CAgggTCAAACTTCCAgTAgAgg 353 64°C/30 s
  1. Bp, base-pairs; TA, annealing temperature; tampl, amplification time.
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Arthritis Research & Therapy

ISSN: 1478-6362

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