Table 1 PCR primer sequences
Gene product | Sequences | Accession number | Position | Product (base-pairs) |
|---|---|---|---|---|
Collagen I(a2) | Up: 5'-CTGGTCCTTCTGGTCCTGTTG | NM_000089 | 3,413 | |
Low: 5'-GTGCGAGCTGGGTTCTTTCTA | 3,957 | 544 | ||
Collagen II(a1) | Up: 5'-CTGGCTCCCAACACTGCCAACGTC | NM_033150 | 4,070 | |
Low: 5'-TCCTTTGGGTTTGCAACGGATTGT | 4,483 | 413 | ||
Collagen X | Up: 5'-ACCCAAGAGGTGCCCCTGGAATAC | NM_000493.2 | 1,416 | |
Low: 5'-CCTGAGAAAGAGGAGTGGACATAC | 2,117 | 701 | ||
Aggrecan | Up:5'-AGCTGGGTTCGGGGCATCT | NM_013227 | 6,039 | |
Low:5'-TGGTAGTCTTGGGCATTGTTGTTGA | 6,839 | 800 | ||
IL-1β | Up:5'-ATGGCAGAAGTACCTAAGCTCGC | NM_000576 | 87 | |
Low:5'-ACACAAATTGCATGGTGAAGTCAGTT | 889 | 802 | ||
ALK-1 | Up: 5'-CGGCTCCCTCTACGACTTTCT | Z_22533 | 1,128 | |
Low: 5'-CAGCACTCCCGCATCATCT | 1,479 | 570 | ||
GAPDH | Up: 5'-TGAAGGTCGGAGTCAACGGATTTGGT | NM_002046 | 113 | |
Low: 5'-CATGTGGGCCATGAGGTCCACCAC | 1,095 | 983 |