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Table 1 PCR primer sequences

From: Comparison of marker gene expression in chondrocytes from patients receiving autologous chondrocyte transplantation versus osteoarthritis patients

Gene product Sequences Accession number Position Product (base-pairs)
Collagen I(a2) Up: 5'-CTGGTCCTTCTGGTCCTGTTG NM_000089 3,413  
  Low: 5'-GTGCGAGCTGGGTTCTTTCTA   3,957 544
Collagen II(a1) Up: 5'-CTGGCTCCCAACACTGCCAACGTC NM_033150 4,070  
  Low: 5'-TCCTTTGGGTTTGCAACGGATTGT   4,483 413
Collagen X Up: 5'-ACCCAAGAGGTGCCCCTGGAATAC NM_000493.2 1,416  
  Low: 5'-CCTGAGAAAGAGGAGTGGACATAC   2,117 701
Aggrecan Up:5'-AGCTGGGTTCGGGGCATCT NM_013227 6,039  
  Low:5'-TGGTAGTCTTGGGCATTGTTGTTGA   6,839 800
IL-1β Up:5'-ATGGCAGAAGTACCTAAGCTCGC NM_000576 87  
  Low:5'-ACACAAATTGCATGGTGAAGTCAGTT   889 802
ALK-1 Up: 5'-CGGCTCCCTCTACGACTTTCT Z_22533 1,128  
  Low: 5'-CAGCACTCCCGCATCATCT   1,479 570
GAPDH Up: 5'-TGAAGGTCGGAGTCAACGGATTTGGT NM_002046 113  
  Low: 5'-CATGTGGGCCATGAGGTCCACCAC   1,095 983
  1. ALK = activin-like kinase; GAPDH = glyceraldehyde-3-phosphate dehydrogenase.