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Table 1 Sequences of PCR primers used

From: Reduction of urate crystal-induced inflammation by root extracts from traditional oriental medicinal plants: elevation of prostaglandin D2levels

Target gene

Sequence

GAPDH forward

5'TGCAGTGGCAAAGTGGAGATT3'

GAPDH reverse

5'ATTTGCCGTGAGTGGAGTCAT3'

IL-6 forward

5'GGAGAGGAGACTTCACAG3'

IL-6 reverse

5'GCCATTGCACAACTCTTTTC3'

TNF-α forward

5'CATCTTCTCAAAATTCGAGTGACAA3'

TNF-α reverse

5'TGGGAGTAGACAAGGTACAACCC3'

h-PGDS forward

5'ATCCAAGGCTGGTGACTTTACG3'

h-PGDS reverse

5'TGAAGGCAACATGGATCAGCTA3'

  1. GAPDH, glyceraldehyde 3-phosphate dehydrogenase; h-PGDS, hematopoietic prostaglandin D synthase; IL, interleukin; PCR, polymerase chain reaction; TNF, tumor necrosis factor.
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Arthritis Research & Therapy

ISSN: 1478-6362

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