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Table 3 Summary of effects of the root extracts*

From: Reduction of urate crystal-induced inflammation by root extracts from traditional oriental medicinal plants: elevation of prostaglandin D2levels

Parameter Assay Change No. of mice per group
Leukocyte count, exudate Cell count -87% 10
Neutrophil density, membrane Cell count -68% 4
IL-6 protein, exudate ELISA -50% 7
IL-6 mRNA, membrane qRT-PCR -100% 4 + 4**
TNF-α mRNA, membrane qRT-PCR -100% 4 + 4**
PGE2, exudate ELISA -69% 7
PGD2, exudate ELISA +5.2-fold 7
Ratio of PGD2:PGE2 ELISA +9.0-fold 7
h-PGDS mRNA, membrane qRT-PCR +3.7-fold 5
  1. * Compared with MSU-stimulated pouches from mice fed water. All percentage differences were significant at p < 0.05.
  2. **Duplicate experiments. ELISA, enzyme-linked immunosorbent assay; h-PGDS, hematopoietic prostaglandin D synthase; IL, interleukin; MSU, monosodium urate; PGD2, prostaglandin D2; PGE2, prostaglandin E2; qRT-PCR, relative quantitative reverse transcriptase polymerase chain reaction; TNF, tumor necrosis factor.