Reduction of urate crystal-induced inflammation by root extracts from traditional oriental medicinal plants: elevation of prostaglandin D2levels

Table 3 Summary of effects of the root extracts*

Parameter	Assay	Change	No. of mice per group
Leukocyte count, exudate	Cell count	-87%	10
Neutrophil density, membrane	Cell count	-68%	4
IL-6 protein, exudate	ELISA	-50%	7
IL-6 mRNA, membrane	qRT-PCR	-100%	4 + 4**
TNF-α mRNA, membrane	qRT-PCR	-100%	4 + 4**
PGE₂, exudate	ELISA	-69%	7
PGD₂, exudate	ELISA	+5.2-fold	7
Ratio of PGD₂:PGE₂	ELISA	+9.0-fold	7
h-PGDS mRNA, membrane	qRT-PCR	+3.7-fold	5

* Compared with MSU-stimulated pouches from mice fed water. All percentage differences were significant at p < 0.05.
**Duplicate experiments. ELISA, enzyme-linked immunosorbent assay; h-PGDS, hematopoietic prostaglandin D synthase; IL, interleukin; MSU, monosodium urate; PGD₂, prostaglandin D₂; PGE₂, prostaglandin E₂; qRT-PCR, relative quantitative reverse transcriptase polymerase chain reaction; TNF, tumor necrosis factor.

ISSN: 1478-6362