Figure 3

Treatment with anti-JAM-C antibody decreases the severity of antigen-induced arthritis. (a) Joint inflammation on days 1, 3, and 7 after intra-articular methylated bovine serum albumin (mBSA) injection. Results are expressed as the ratio of technetium-99m (^99mTc) uptake in the arthritic over the non-inflamed knee. The mean ± standard error of the mean (SEM) of the ratios is shown for anti-JAM-C antibody-treated (n = 10 for days 1 and 3, n = 6 for day 7; black symbols) and isotype-matched control antibody-treated (n = 10 for days 1 and 3, n = 5 for day 7; open symbols) mice. Joint inflammation was significantly reduced in anti-JAM-C antibody-treated mice on day 3. (b) Representative histological sections for anti-JAM-C antibody-treated (upper panel) and isotype-matched control antibody-treated (lower panel) mice 8 days after intra-articular mBSA injection (original magnification × 40, scale bar = 125 μm). Arrowheads: cartilage erosions; asterisks: pannus; broken lines: synovial thickness. (c) Histological scores shown as the mean ± SEM for anti-JAM-C antibody-treated (n = 6, black columns) and isotype-matched control antibody-treated (n = 5, open columns) mice 8 days after intra-articular mBSA injection. Synovial inflammation was significantly reduced in anti-JAM-C antibody-treated mice. (d) Circulating levels of serum amyloid A (SAA) on days 4 and 8 after intra-articular mBSA injection. Results shown represent the mean ± SEM for anti-JAM-C antibody-treated (n = 5 on day 4, n = 6 on day 8, black columns) and isotype-matched control antibody-treated (n = 5 at both time points, open columns) mice. Serum SAA levels were significantly decreased in anti-JAM-C antibody-treated mice on day 4. *p < 0.05 versus control mice, as assessed by analysis of variance. Ab, antibody; JAM-C, junctional adhesion molecule-C.