The cytokine response to mycobacterial hsp65 (Bhsp65) of lymph node cells (LNCs) of Celastrus-fed versus water-fed Lewis (LEW) rats. Two groups of LEW rats (n = 6 to 9) were fed either Celastrus (3 g/kg) or water as described in the legend to Figure 1. A sub-group of these LEW rats was euthanized on day 12 after M. tuberculosis H37Ra immunization, and the draining LNCs of these rats were cultured in a 96-well plate in the presence of the indicated recall antigens (HEL, hen eggwhite lysozyme; B177, synthetic peptide 177–191 of Bhsp65). The supernatant was collected after 72 h of cell culture and tested in ELISA for (a) IFN-γ and (b) IL-10. The results are expressed as pg/ml (mean + standard error of the mean). The difference in the level of IL-10 but not of IFN-γ in response to Bhsp65 in Celastrus-fed versus water-fed rats is statistically significant (*p < 0.05). The mean IFN-γ/IL-10 ratio in response to Bhsp65 of Celastrus-treated (8.79) rats was significantly reduced compared to that of the water-fed (20.76) rats.