| | | | | Hybridoma response (SI) |
|---|
Compound | HC gp-39 | Sequence | HLA-DR binding IC50 (μM) | 5G11 | 8B12 | 14G11 | 20H5 |
|---|
| | | | | Vβ10b | Vβ10b | Vβ6 | Vβ10b |
1 | 263–275 | RSFTLASSETGVG | 0.12 |
13
|
9
|
15
|
26
|
2 | 263–274 |
RSFTLASSETGV
| 0.09 |
7
|
8
|
10
|
22
|
3 | 263–273 |
RSFTLASSETG
| 0.20 | 2 |
5
|
5
|
15
|
4 | 263–272 |
RSFTLASSET
| 7.0 | 1 | 1 | 1 | 2 |
5 | 264–275 |
SFTLASSETGVG
| 0.09 |
12
|
10
|
15
|
27
|
6 | 265–275 |
FTLASSETGVG
| 0.11 |
11
|
6
|
8
|
24
|
7 | 266–275 |
TLASSETGVG
| >100 | 1 | 1 | 1 |
5
|
8 | 267–275 |
LASSETGVG
| >100 | 1 | 1 | 1 | 1 |
9 | 264–274 |
SFTLASSETGV
| 0.12 | ND |
7
|
13
|
22
|
- Binding of peptides to HLA-DR4(B1*0401) was determined in a competition binding assay. IC50 = 50% inhibitory concentration. IC50 < 1 μM = high affinity binder; IC50 between 1 and 10 μM = good binder; IC50 between 10 and 100 μM = intermediate binder; IC50 between 100 and 1,000 μM = poor binder. The decimals do not reflect the accuracy of the data but represent the calculated geometric means obtained in two experiments. The HLA-DRB1*0401 expressing B lymphoblastoid cell line was used as a source of antigen-presenting cells. Background values for hybridomas 5G11, 8B12, 14G11 and 20H5 were 8,147, 7,470, 8,598 and 35,749 mean fluorescence units/counts, respectively. Hybridoma IL-2 response is expressed as stimulation index (SI) calculated as the ratio of mean fluorescence counts of antigen-stimulated cultures and control cultures. Values in bold are considered positive (SI > 2). Data are representative of at least three independent experiments. ND, not determined.
