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Table 3 Antigen-specific response (proliferation and cytokine production) of popliteal lymph node cells from HC gp-39(263–275)-immunized huCD4, HLA-DR4 transgenic mice to single site substituted altered peptide ligands

From: Identification of an altered peptide ligand based on the endogenously presented, rheumatoid arthritis-associated, human cartilage glycoprotein-39(263–275) epitope: an MHC anchor variant peptide for immune modulation

      Concentration (pg/ml)
Compound HC gp39 Sequence [Peptide] (μg/ml) Prol (SI) MIP1α RANTES IFNγ
1 263–275 RSFTLASSETGVG 10 8 252 23 16
    2 6 128 13 6
    0.4 3 25 6 <5
12 F265A3 RSATLASSETGVG 10 3 27 9 <5
    2 1 <24 <6 <5
13 T266A4 RSFALASSETGVG 10 7 116 18 9
    2 4 47 11 <5
14 L267A5 RSFTAASSETGVG 10 7 295 32 14
    2 6 112 11 <5
15 A268D6 RSFTLDSSETGVG 10 3 29 <6 <5
    2 1 <24 <6 <5
16 S269A7 RSFTLAASETGVG 10 2 <24 <6 <5
    2 2 <24 <6 <5
17 S270A8 RSFTLASAETGVG 10 7 279 20 12
    2 5 86 12 <5
18 E271A9 RSFTLASSATGVG 10 1 <24 <6 <5
    2 1 <24 <6 <5
19 T272A10 RSFTLASSEAGVG 10 1 <24 <6 <5
    2 1 <24 <6 <5
20 G273A11 RSFTLASSETAVG 10 4 47 15 <5
    2 1 <24 <6 <5
   No peptide 0 1 <24 <6 <5
  1. Antigen-specific responses (proliferation and cytokine production) were assessed at 96 h. Proliferation is expressed as stimulation index (SI; antigen specific cpm/background cpm). Background was 407 cpm. The standard deviation of measurements did not exceed 30%. For the proliferative assay an SI > 2 (bold) is regarded as positive. Cytokine production of Mip1α, RANTES and IFNγ is expressed in pg/ml. The limit of detection for these cytokines is 24, 5 and 6 pg/ml respectively. Values in bold are regarded as positive. Other cytokines assayed (such as IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-10, IL-12p40, IL-12p70, IL-17, granulocyte colony-stimulating factor, granulocyte-macrophage colony-stimulating factor, tumor necrosis factor-α and cytokine-induced neutrophil chemoattractant (KC)) were not detected. Prol, proliferation.