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Table 3 Antigen-specific response (proliferation and cytokine production) of popliteal lymph node cells from HC gp-39(263–275)-immunized huCD4, HLA-DR4 transgenic mice to single site substituted altered peptide ligands

From: Identification of an altered peptide ligand based on the endogenously presented, rheumatoid arthritis-associated, human cartilage glycoprotein-39(263–275) epitope: an MHC anchor variant peptide for immune modulation

     

Concentration (pg/ml)

Compound

HC gp39

Sequence

[Peptide] (μg/ml)

Prol (SI)

MIP1α

RANTES

IFNγ

1

263–275

RSFTLASSETGVG

10

8

252

23

16

   

2

6

128

13

6

   

0.4

3

25

6

<5

12

F265A3

RSATLASSETGVG

10

3

27

9

<5

   

2

1

<24

<6

<5

13

T266A4

RSFALASSETGVG

10

7

116

18

9

   

2

4

47

11

<5

14

L267A5

RSFTAASSETGVG

10

7

295

32

14

   

2

6

112

11

<5

15

A268D6

RSFTLDSSETGVG

10

3

29

<6

<5

   

2

1

<24

<6

<5

16

S269A7

RSFTLAASETGVG

10

2

<24

<6

<5

   

2

2

<24

<6

<5

17

S270A8

RSFTLASAETGVG

10

7

279

20

12

   

2

5

86

12

<5

18

E271A9

RSFTLASSATGVG

10

1

<24

<6

<5

   

2

1

<24

<6

<5

19

T272A10

RSFTLASSEAGVG

10

1

<24

<6

<5

   

2

1

<24

<6

<5

20

G273A11

RSFTLASSETAVG

10

4

47

15

<5

   

2

1

<24

<6

<5

  

No peptide

0

1

<24

<6

<5

  1. Antigen-specific responses (proliferation and cytokine production) were assessed at 96 h. Proliferation is expressed as stimulation index (SI; antigen specific cpm/background cpm). Background was 407 cpm. The standard deviation of measurements did not exceed 30%. For the proliferative assay an SI > 2 (bold) is regarded as positive. Cytokine production of Mip1α, RANTES and IFNγ is expressed in pg/ml. The limit of detection for these cytokines is 24, 5 and 6 pg/ml respectively. Values in bold are regarded as positive. Other cytokines assayed (such as IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-10, IL-12p40, IL-12p70, IL-17, granulocyte colony-stimulating factor, granulocyte-macrophage colony-stimulating factor, tumor necrosis factor-α and cytokine-induced neutrophil chemoattractant (KC)) were not detected. Prol, proliferation.